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KMID : 1234420090370040350
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Korean Journal of Microbiololgy and Biotechnology
2009 Volume.37 No. 4 p.350 ~ p.354
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Identification and Characterization of Alginate Lyase Producing Pseudomonas sp. N7151-6.
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Lee Jae-Hyung
Bae Min-Ji
Kim Yang-Chun
Nam Soo-Wan
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Abstract
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A Gram-negative, alginate lyase-producing bacterium was isolated from the Haeundae Coast, Korea. The isolated strain N7151-6 produced alginate lyase. The optimal temperature and pH for growth were found to be 30oC and pH 8.0, respectively. This strain can be grown at the NaCl concentration of 0-7% (w/v). Analysis of 16S rDNA sequence and physiological profiling indicated that the strain N7151-6 belonged to Pseudomonas sp. The enzyme alginate lyase produced by Pseudomonas sp. N7151-6 was partially purified by ultrafiltration (MWCO= 30 kDa). The optimum pH and temperature for the activity of the purified enzyme were found to be 7.0 and 30oC, respectively. The enzyme was stable at the pH range of 5.0-9.0 and temperature range of 23-37oC. The total activity of alginate lyase produced was reached about 110 unit/L.
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KEYWORD
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Alginate lyase, Pseudomonas sp., 16S rDNA, physiological reaction profiling
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